|
A1 根瘤菌培养基：
| K2HPO4 |
0.5g |
1％Na2MoO4·2H2O |
2mL |
| MgSO4·7H2O |
0.2g |
1％H3BO3 |
2mL |
| NaCl |
0.1g |
酵母膏1.0g(或酵母粉0.8g) |
| C6H14O6 |
10g |
0.5％刚果红 |
5mL |
| CaCO3 |
1.5g |
pH |
7.0 |
A2 固氮菌用阿须贝氏(Ashby)培养基：
| KH2PO4 |
0.2g |
CaCO3 |
5.0g |
| MgSO4·7H2O |
0.2g |
C6H14O6 |
10.0g |
| NaCl |
0.2g |
CaSO4·2H2O |
0.1g |
|
|
pH |
7.0 |
A3 联合固氮菌培养基：
(1)培养基：
| V─CeH11O7Na |
5g |
NaCl |
0.1g |
| KH2PO4 |
0.4g |
CaCl2 |
0.02g |
| K2HPO4 |
0.1g |
FeCl3 |
0.01g |
| MgSO4·7H2O |
0.2g |
Na2MoO4·2H2O |
0.002g |
| 酵母膏 |
1.0g |
pH |
6.8～7.0 |
(2)培养基：
| C4H4O5Na2 |
5g |
CaCl2 |
0.02g |
| K2HPO4 |
0.1g |
Na2MoO4·2H2O |
0.002g |
| KH2PO4 |
0.4g |
FeCl3 |
0.01g |
| MgSO4·7H2O |
0.2g |
BTB(0.5g/L) |
5mL |
| NaCl |
0.1g |
pH |
7.0 |
A4 硅酸盐细菌培养基：
| C12H22O11 |
5.0g |
Na2HPO4 |
2.0g |
| MgSO4·7H2O |
0.5g |
FeCl3 |
0.005g |
| CaCO3 |
0.1g |
玻璃粉 |
1.0g |
|
|
pH |
7.0 |
A5 解磷细菌培养基：
(1)有机磷细菌培养基：
| C6H12O6·H2O |
10g |
(NH4)2SO4 |
0.5g |
| NaCl |
0.3g |
KCl |
0.3g |
| MgSO4·7H2O |
0.3g |
FeSO4·7H2O |
0.03g |
| MnSO4·4H2O |
0.03g |
CaCO3 |
5g |
| 卵磷脂 |
0.2g |
pH |
7.0～7.5 |
(2)无机磷细菌培养基：
| C6H12O6·H2O |
10g |
(NH4)2SO4 |
0.5g |
| NaCl |
0.3g |
KCl |
0.3g |
| MgSO4·7H2O |
0.3g |
FeSO4·7H2O |
0.03g |
| MnSO4·4H2O |
0.03g |
Ca3(PO4)2 |
5g |
|
|
pH |
7.0～7.5 |
A6 马丁培养基(测霉菌数)
| KH2PO4 |
1.0g |
C6H12O6·H2O |
10.0g |
| MgSO4·7H2O |
0.5g |
蛋白胨 |
5.0g |
| 1％孟加拉红水溶液3.3mL |
|
|
|
A7 放线菌培养基(改良高氏1号)
| KNO3 |
1.0g |
FeSO4.7H2O |
0.01g |
| KH2PO4 |
0.5g |
可溶性淀粉(先加少量冷水调成糊状加入) |
20.0g |
| MgSO4.7H2O |
0.5g |
|
|
| NaCl |
0.5g |
pH |
7.2～7.4 |
临用时在已融化的高氏1号培养基中加入重铬酸钾溶液，以抑制细菌和霉菌生长。每300mL培养基中加3％重铬酸钾1mL(100mg/kg)。
注：以上各培养基均需加入蒸馏水1000mL，琼脂18～20g。
A8 刚果红染液(0.5％)
| 刚果红(Congored) |
0.5g |
| 蒸馏水 |
100mL |
|
