Column:  ODS-2

Eluent: A: H₂0 + 0.5% H₃PO₄  B: MeCN

Gradient: 18%B for 4 min
18%B to 45%B in 100 min
Hold at 45%B for 5 min

Flow: 1.0 ml/min

Temp:  

Detection: UV @ 220nm