-
Column: ODS-3V
-
Eluent: MeCN:10mM NaH2PO4 +10 mM citric acid (11:89)
-
Gradient: None
-
Flow: 1.0 ml/min
-
Temp: 30?/strong>
-
Detection: UV @ 302nm
|

1.folic acid;2.methotrexate |
|
-
Column: ODS-2
-
Eluent: A: 5mM SPS+ 0.1% H3PO4
B: Eluent "A" in 80% MeCN
-
Gradient: 2.5%B to 80%B in 20 minutes
-
Flow: 1.0 ml/min
-
Temp:
-
Detection: UV @ 254nm
1.L-ascorbic acid;2.nicotinic acid;3.nicotinamide;4.pyridoxal
5.pyridoxamine;6.pyridoxine;7.thiamine;8.folic
acid;9.cyanocobalamine;10.riboflavin |
 |
|
Column: ODS-2
Eluent: MeCN:50mM KH2PO4 (pH 4 with H3PO4) 10:90
Gradient: None
Flow: 1.0 ml/min
Temp: 40?/font>
Detection: UV @ 210nm
色谱峰:
1.nicotinic acid;2.nicotinic acid amide;3.pantothenic
acid;4.folic acid
5.unknown;6.D-biotin;7.riboflavin |
 |
|
|
Column: ODS-3V
Eluent: MeCN:0.2% H3PO4 + 5mM IPCC-05 10:90
Gradient: None
Flow: 1.0 ml/min
Temp: 40?/font>
Detection: UV @ 210nm
色谱峰:
1.niacin;2.nicotinamide ;3.calcium
pantothenate
4.folic acid;5.B12;6.B2
|
 |
|
|
|